This exceptional museum is located in the former Dominican convent that dates back to the 13th century, with a beautifully preserved double-arcaded cloister. In 2015, the museum expanded its gallery space with an adjoining building that occupies the town's former municipal baths. 

The Unterlinden Museum has an extensive collection of Romanesque and Gothic sculpture, medieval paintings, folk art, and crafts. The first floor is devoted to Alsatian toys, furniture, and decorative objects (porcelain, faience, stained glass, and prints).

 One of the highlights of the museum is the Isenheim Chapel, which contains fine paintings by early German artists, including Passion scenes by Martin Schongauer and works by Isenmann and Lucas Cranach the Elder. 

The chief treasure of the chapel is Matthias Grünewald's Isenheim Altar, one of the most moving masterpieces of German painting. This imaginative work of glowing color was painted around 1515 for the convent of Isenheim near Guebwiller.

 The museum also has a lapidary collection, as well as religious art. The museum's contemporary art collection includes Picasso, Léger, Rouault, Mathieu, Vasarely, and Braque